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Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations Article

Lanqing Liu, Hong Liu, Wei Zhang, Mingdong Yao, Bingzhi Li, Duo Liu, Yingjin Yuan

Engineering 2019, Volume 5, Issue 2,   Pages 287-295 doi: 10.1016/j.eng.2018.11.029

Abstract: presents several challenges, especially when the expression and activation of the plant cytochrome P450 enzymein Saccharomyces cerevisiae to take on a role similar to that of the plant-derived cytochrome P450 enzymeThe highest production of caffeic acid was obtained with the enzyme combination of HpaB from PseudomonasThe compatibility of heterologous enzymes within a yeast chassis was effectively improved, as the caffeicPseudomonas aeruginosa were distinguished from those other HpaBs, and might play critical roles in affecting enzyme

Keywords： Saccharomyces cerevisiae     Caffeic acid     Heterologous enzyme     Cytochrome P450     Synthetic biology    

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Designer enzyme for green materials innovation: Lactate-polymerizing enzyme as a key catalyst

Seiichi Taguchi

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 139-142 doi: 10.1007/s11705-017-1636-0

Abstract: In this commentary, discovery story of a “lactate-polymerizing enzyme” (LPE)?and LPE-based?

Keywords： synthetic biology     enzyme evolutionary engineering     polyhydroxyalkanoate    

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Profiling influences of gene overexpression on heterologous resveratrol production in

Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 117-125 doi: 10.1007/s11705-016-1601-3

Abstract: Metabolic engineering of heterologous resveratrol production in faces challenges as the precursor L-tyrosine

Keywords： resveratrol     aromatic amino acid     DNA assembly     metabolic engineering     gene overexpression    

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Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 1,   Pages 89-94 doi: 10.1007/s11783-007-0017-1

Abstract: The present work studied the influence of glucose feeding on the ligninolytic enzyme production of inthe first day of the culture (24 h after the inoculation) stimulated both fungal biomass growth and enzymeof MnP activity were 2.7 and 3 times greater compared to the contrast culture, respectively, and the enzyme

Keywords： enzyme production     fed-batch culture     nitrogen-limited     inoculation     mmol/L    

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Diffusion process in enzyme–metal hybrid catalysts

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 6,   Pages 921-929 doi: 10.1007/s11705-022-2144-4

Abstract: Enzyme–metal hybrid catalysts bridge the gap between enzymatic and heterogeneous catalysis, which isPrevious studies have demonstrated that the enzyme–metal hybrid catalysts exhibited considerably highercatalytic efficiency in cascade reactions, compared with that of the combination of separated enzymeHere, we investigated the diffusion process in enzyme–metal hybrid catalysts using Pd/lipase-Pluronicand metal active sites, which provides a theoretical foundation for the rational design of enzyme–metal

Keywords： enzyme–metal hybrid catalyst     internal diffusion     proximity effect     kinetic model    

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Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 3,   Pages 381-384 doi: 10.1007/s11783-007-0065-6

Abstract: This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA

Keywords： conventional     sandwich     pretreatment     enzyme-linked immunosorbent     detection    

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Enzyme@bismuth-ellagic acid: a versatile platform for enzyme immobilization with enhanced acid-base stability

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 6,   Pages 784-794 doi: 10.1007/s11705-022-2278-4

Abstract: In situ encapsulation is an effective way to synthesize enzyme@metal–organic framework biocatalysts

Keywords： bismuth-ellagic acid     in situ encapsulation     enzyme@MOF biocomposites    

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Soil enzyme activities and their indication for fertility of urban forest soil

SHAN Qihua, YU Yuanchun, ZHANG Jinchi, YU Jian

Frontiers of Environmental Science & Engineering 2008, Volume 2, Issue 2,   Pages 218-223 doi: 10.1007/s11783-008-0037-5

Abstract: To reveal the biological characteristics of urban forest soil and the effects of soil enzyme on soilfertility as well as the correlation between physicochemical properties and enzyme activities, 44 urbanBasic soil physicochemical properties and enzyme activities were analyzed in the laboratory.Both in 0–20 cm and 20–40 cm soil, soil enzyme system (hydrogen peroxidase, dehydrogenase, alkaline phosphataseThe more soil enzyme activities there were, the higher the fertility of urban forest soil.
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Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

Jongmoon Park,Yunnam Choi

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 66-71 doi: 10.1007/s11705-016-1591-1

Abstract: insufficient supply of NADH, tends to limit their production yields in case of utilizing NADH-dependent enzyme

Keywords： NADH-dependent enzyme     NADPH-dependent enzyme     transhydrogenase     site-directed mutagenesis     enzyme engineering    

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Heterologous expression of

Di Li,Swati Yewalkar,Xiaotao Bi,Sheldon Duff,Dusko Posarac,Heli Wang,Layne A. Woodfin,Jan-Hendrik Hehemann,Sheila C. Potter,Francis E. Nano

Frontiers of Environmental Science & Engineering 2017, Volume 11, Issue 2, doi: 10.1007/s11783-017-0910-1

Abstract: The maximum enzyme activity was observed at a pH of 8.0 and a temperature of 70°C.0.083 h , a biomass productivity of 0.42 g·L ·d , a biomass concentration of 3.697 g·L , and a specific enzymeAll parameters decreased as CO2 concentration increased from 5% to 10% and further to 15% CO2, except enzymereflected by the biomass productivity (0.26 g·L ·d ), biomass concentration (2.416 g·L ), and specific enzyme

Keywords： Synechococcus sp. PCC 7002     Thermotoga maritima     LamA gene     Endo-β-1     3-glucanase     CO2 fixation    

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Plant diversity reduces the effect of multiple heavy metal pollution on soil enzyme activities and microbial

Yang GAO, Chiyuan MIAO, Jun XIA, Liang MAO, Yafeng WANG, Pei ZHOU

Frontiers of Environmental Science & Engineering 2012, Volume 6, Issue 2,   Pages 213-223 doi: 10.1007/s11783-011-0345-z

Abstract: diversity could reduce the impacts of multiple heavy metal pollution on soil microbial structure and soil enzymeSoil enzyme activity was higher in phytoremediated soil than in planted soil in this study.

Keywords： enzyme activity     soil DNA     microbial population     plant diversity     heavy metal    

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Degradation of 4-aminophenol by hydrogen peroxide oxidation using enzyme from Serratia marcescens as

SUN Min, YOU Yahua, DENG Shengsong, GAO Wenxia, YAO Risheng

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 1,   Pages 95-98 doi: 10.1007/s11783-007-0018-0

Abstract: Also, the location of the enzyme in the cell, which could catalyze the degradation of 4-aminophenol,The enzyme that could catalyze the degradation of 4-aminophenol was mainly extracellular enzyme.

Keywords： degradation process     temperature     effecting     degradation     volume    

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Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay

SHENG Jianwu, HE Miao, YU Shaoqing, SHI Hanchang, QIAN Yi

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 3,   Pages 329-333 doi: 10.1007/s11783-007-0056-7

Abstract: In this paper, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established andg/mL, the dilution of monoclonal antibody MC10E7 was 1:3 000, the dilution of enzyme tracer (goat anti-mouse

Keywords： o-phenylenediamine     4-arginine     ic-ELISA     substrate     chromatography    

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Enzyme-instructed self-assembly of peptides containing phosphoserine to form supramolecular hydrogels

Jie Zhou, Xuewen Du, Jiaqing Wang, Natsuko Yamagata, Bing Xu

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 509-515 doi: 10.1007/s11705-017-1613-7

Abstract: Enzyme-instructed self-assembly (EISA) offers a facile approach to explore the supramolecular assemblies

Keywords： enzyme-instructed self-assembly     phosphoserine     phosphatase     supramolecular hydrogel    

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Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice

ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong

Frontiers of Medicine 2008, Volume 2, Issue 1,   Pages 95-99 doi: 10.1007/s11684-008-0017-y

Abstract: Schistosomiasis japonica, a zoonosis caused by , is endemic to the Philippines and China. Several vaccine candidates have been identified and tested in different animal models, but it is still unclear which will be optimal for testing in the field. Therefore, new antigens and strategies are necessary for vaccine development against schistosomiasis japonica. The Sj14-3-3 gene was amplified and subcloned into the expression vector pPICZ?-B and transformed into X-33 by electroporation. Three transformants were induced with methanol. The cultural supernatant was collected and tested by SDS-PAGE and Western blotting. The protein of rSj14-3-3 was prepared and purified and BALB/c mice were immunized which was followed by a challenging infection. The immuno-protection was then evaluated. The Sj14-3-3 gene was expressed and secreted into the medium and its molecular weight was about 35000 as determined by SDS-PAGE. Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity. The results of the immuno-protective experiments revealed that the worm reduction was 26.0%, 32.2%, and 36.8%, respectively. The number of eggs in liver tissue was reduced by 36.8%, 43.2%, and 46.1%, respectively. The recombinant Sj14-3-3 of eukaryotic expression in was successfully harvested. The molecular vaccine of Sj14-3-3 could partially induce resistance to the infection with in BALB/c mice. The recombinant protein Sj14-3-3 has promising immunological potentials for further approach to the diagnosis and development of molecular vaccine.

Keywords： development     challenging     rSj14-3-3     resistance     cultural supernatant    

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Title Author Date Type Operation

Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations

Lanqing Liu, Hong Liu, Wei Zhang, Mingdong Yao, Bingzhi Li, Duo Liu, Yingjin Yuan

Journal Article

Designer enzyme for green materials innovation: Lactate-polymerizing enzyme as a key catalyst

Seiichi Taguchi

Journal Article

Profiling influences of gene overexpression on heterologous resveratrol production in

Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan

Journal Article

Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

Journal Article

Diffusion process in enzyme–metal hybrid catalysts

Journal Article

Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

Journal Article

Enzyme@bismuth-ellagic acid: a versatile platform for enzyme immobilization with enhanced acid-base stability

Journal Article

Soil enzyme activities and their indication for fertility of urban forest soil

SHAN Qihua, YU Yuanchun, ZHANG Jinchi, YU Jian

Journal Article

Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

Jongmoon Park,Yunnam Choi

Journal Article

Heterologous expression of

Di Li,Swati Yewalkar,Xiaotao Bi,Sheldon Duff,Dusko Posarac,Heli Wang,Layne A. Woodfin,Jan-Hendrik Hehemann,Sheila C. Potter,Francis E. Nano

Journal Article

Plant diversity reduces the effect of multiple heavy metal pollution on soil enzyme activities and microbial

Yang GAO, Chiyuan MIAO, Jun XIA, Liang MAO, Yafeng WANG, Pei ZHOU

Journal Article

Degradation of 4-aminophenol by hydrogen peroxide oxidation using enzyme from Serratia marcescens as

SUN Min, YOU Yahua, DENG Shengsong, GAO Wenxia, YAO Risheng

Journal Article

Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay

SHENG Jianwu, HE Miao, YU Shaoqing, SHI Hanchang, QIAN Yi

Journal Article

Enzyme-instructed self-assembly of peptides containing phosphoserine to form supramolecular hydrogels

Jie Zhou, Xuewen Du, Jiaqing Wang, Natsuko Yamagata, Bing Xu

Journal Article

Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice

ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong

Journal Article